Details
Description: Mouse monoclonal antibody to human CA 19-9 antigen.
Purification: Protein G affinity purified
Product Type: Primary antibody detection
Target Protein: Human CA 19-9
Immunogen: Human colon adenocarcinoma cell line SW1116
Fusion Myeloma: Sp2/0-Ag14
Specificity: Reactive with SW1116 and also reactive with highly purified human CA19-9 antigen from the cell culture supernatant of a human Colon adenocarcinoma.
Reactivity: Human, others not tested
Species Reactivity: Not available
Host / Isotype: Mouse, IgG1 Kappa
Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.2
Reconstitution: Double distilled water is recommended to reconstitute the antibody. Avoid repeated freeze and thaw cycles.
Storage: Store at -20oC
Research Area： Oncology
Background: CA19-9 has been the most valuable serum biomarker in monitoring progress and regression of pancreatic cancer and colorectal cancer. CA19-9, also known as sialyl-Lewisᴬ, is a tetrasaccharide which is expressed in abundance on the surface of cancer cells. CA 19-9 usually ranges from 0 to 37 units per milliliter in a healthy person. CA 19-9 levels can be higher in patients with pancreatic cancer and other cancers.
Applications:
Indirect ELISA: CA19-9 mAb clone 9B2 at a wide concentration range can react with ELISA plate coated with SW1116 and also with ELISA plate coated with purified CA19-9 at 0.125 KU per well.
Sandwich ELISA: CA19-9 mAb clone 9B2 can be used as detection antibody in Sandwich ELISA when anti CA19-9 clone 4E11 (Cat. No.: MO-T40015D) is used as capture antibody.
Immunohistochemistry (IHC): CA19-9 mAb clone 9B2 was used to stain pancreatic cancer tissue and paracancerous tissue. Briefly, the paraffin sections of pancreatic cancer tissue and paracancerous tissue were treated with EDTA to re-naturalize antigen epitopes and with 3% H2O2 to remove endogenous catalase. The sections were blocked with goat serum, incubated with 1:500 diluted CA19-9 mAb 9B2 overnight at 4℃ and washed, followed by incubation with HRP-conjugated goat anti-mouse IgG. Sections were stained with DAB and counter-stained with Hematoxylin.
References: If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
