T4 RNA Ligase 2, i.e., T4 RNA ligase 2, is an ATP-dependent double-stranded RNA ligase (dsRNA ligase), also known as T4 Rnl2, which can be used for intramolecular circularization and intermolecular linear ligation of double-stranded RNA. Unlike T4 RNA Ligase 1 (R0502), T4 RNA Ligase 2 exhibits much higher ligation activity on nicks in double-stranded RNA than on the ends of single-stranded RNA.
T4 RNA Ligase 2 can also be used to ligate 3′ hydroxyl groups of RNA strands and 5′ phosphate groups of DNA strands within or between double-stranded nucleic acids (double-stranded RNA, RNA/DNA hybrids, or double-stranded DNA). T4 RNA Ligase 2 requires the presence of a 5′ phosphate and a 3′ hydroxyl for ligation, and the ligation reaction can occur between a 5′ phosphate of an RNA or DNA strand and a 3′ hydroxyl of an RNA strand.
Case Analysis
Under the same reaction system, Gisay BiotechT4 RNA Ligase 2 shows higher actual ligation efficiency compared to competing products; in this reaction system, a noticeable ligation effect is achieved with only 1 U.
Q & A
1. What are the main applications of this kit?
Answer: Mainly used to join nicks in double-strandedRNA (i.e., cohesive-end ligation of double-stranded RNA), and can also be used to seal nicks between an RNA 3′ hydroxyl and a DNA 5′ phosphate within double-stranded structures.
Download
GSPure® T4 RNA Ligase 2 Cat. No. R0503.pdf
