T4 RNA Ligase 1, i.e., T4 RNA ligase 1, is an ATP-dependent enzyme that catalyzes the formation of phosphodiester bonds between 5′-P termini and 3′-OH termini either inter- or intramolecularly in single-stranded RNA, single-stranded DNA, or mononucleotide molecules. It can be used for intermolecular ligation of ssRNA; intermolecular ligation of ssRNA and ssDNA; labeling the 3′-OH end of ssRNA with radioactive isotopes such as 5’-[32P]pCp; synthesis of single-stranded oligo RNA and oligo DNA; special modification of tRNA; incorporation of non-natural amino acids into proteins; and other applications.
Case Analysis
Under the same reaction conditions, Gisay Biotech‘s T4 RNA Ligase 1 shows similar catalytic activity but higher ligation efficiency compared to competing products.
Q & A
1. Which molecules can this kit catalyze to react?
Answer:T4 RNA Ligase 1, namely T4 RNA ligase 1, is an ATP-dependent enzyme that can catalyze the formation of phosphodiester bonds between 5′-P termini and 3′-OH termini inter- or intramolecularly of single-stranded RNA, single-stranded DNA, or mononucleotide molecules. It can be used for intermolecular ligation of ssRNA; intermolecular ligation of ssRNA and ssDNA; labeling of ssRNA 3′-OH termini with radioactive isotopes such as 5’-[32P]pCp; synthesis of single-stranded oligo RNA and oligo DNA; special modification of tRNA; incorporation of non-natural amino acids into proteins
