Applications
This product can be used in any application whereRNase interference may exist to prevent RNA degradation, such as:
1. First-strand cDNA synthesis, RT-PCR (usable in both PCR and qPCR systems);
2. Protection of RNA during in vitro transcription/translation (such as viral in vitro replication systems);
3. Inhibition of RNase activity during RNA isolation and purification processes;
4. RNase protection assays.
This product does not interfere with other commonly used enzymesduring RNA preparation or analytical applications.
Unit definition
InhibitionThe amount of enzyme required to inhibit 50% of the activity of 5 ng RNase A is defined as one activity unit (U). RNase A activity is quantified by inhibition of Cyclic 2′,3′-CMP hydrolysis to 3′-CMP.
Shipping and Storage
Store at -25°C to -18°C, transport with ice packs.
Performance comparison
Figure1. Under the same reaction conditions, GSPure® RNase Inhibitor Murine from Gisae Bio and comparable competitive products are compared, showing a stronger inhibitory effect on RNase A enzymatic activity.
