PPase (Inorganic Pyrophosphatase), i.e., inorganic pyrophosphatase, is an enzyme that catalyzes the conversion of one molecule of pyrophosphate into two molecules of phosphate, with the catalytic reaction as follows:
P2O7-4 + H2O → 2HPO4-2
Inorganic pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. Under natural conditions, this highly exergonic reaction provides the thermodynamic driving force for biosynthetic reactions such as protein,DNA, and RNA synthesis. In reaction systems that generate inorganic pyrophosphate, such as in vitro RNA transcription, DNA replication, and amplification, inorganic pyrophosphatase can prevent accumulation of the byproduct inorganic pyrophosphate, promote the forward reaction, improve reaction efficiency, and increase product yield. The enzyme is a metalloprotein, and its activity is influenced by divalent metal ions, with highest activity in the presence of Mg2+.
Source: Recombinant protein expressed in Escherichia coli
Applications
1. In in vitro transcription, promotes high-yieldRNA synthesis;
2. In nucleic acid amplification reactions such asisothermal amplifications like HDA or LAMP, prevents accumulation of inorganic pyrophosphate, improves reaction efficiency, and increases nucleic acid yield;
3. In reagents using pyrophosphate-based assays forSNP genotyping, removes pyrophosphate contamination.
Shipping and Storage
Transport on dry ice.Store at -25 to -18°C, shelf life two years.
Quality Control
1.No RNase or DNase contamination;
2.Purity by SDS-PAGE >95%.
