In recent years, a large number of circRNA molecules have been discovered in organisms to exert biological functions through various pathways.
Breakthrough research showing that natural circRNAs can serve as translation templates to directly translate peptides or proteins has made it possible to develop circRNA as a carrier for therapeutic drugs; compared with linear RNAs such as mRNA, the closed-loop structure of circRNA greatly prolongs its half-life in the body, and this stable structure is extremely favorable for sustained expression of functional proteins/peptides.
Relying on
more than 10 years of focused R&D and innovative exploration in the circRNA field, Gysai Biotech has established a circRNA bulk production system and can provide off-the-shelf products across multiple series including reporter genes, tumor antigens, anti-tumor, growth factors, immunotherapy, gene editing, protein/antibody replacement, iPSC reprogramming, anti-aging, neurotrophic factors, neural regeneration transformation factors, and natural circular RNAs. The products are prepared using independently owned proprietary technologies, with good integrity and high purity, and can serve as positive controls for experimental exploration, used in translation expression validation, transfection delivery evaluation, process optimization, and other fields to empower next-generation RNA therapeutics.
Product transported on dry ice.Stored at -80℃ ultra-low temperature, can be stably preserved for one year.
Except for circ-EPO, circ-Trp53, circ-TP53, circ-eGFP, circ-Fluc, circ-mCherry and endogenous circular RNAs, all other circRNA off-the-shelf products are tagged with 3xFlag and can have their expression products detected using anti-Flag antibodies.
Case Analysis
1. High integrity
Figure1: Integrity (CE) of purified GSPure® Circ-eGFP (left) and GSPure® Circ-mCherry (right) >95%
2. High purity
Figure2: Purity (HPLC) of purified GSPure® Circ-eGFP (left) and GSPure® Circ-Fluc (right) >90%
3. High quality
Figure3: Fluorescence detection results after transfection of GSPure® Circ-eGFP into 293T cells, magnification: 100×.
Figure4: Fluorescence detection results after transfection of GSPure® Circ-mCherry into 293T cells, magnification: 100×.
Figure5: Luciferase assay results after transfection of GSPure® Circ-Fluc into 293T cells
Figure6: In vivo fluorescence detection 6 h after intramuscular injection of LNP-encapsulated Circ-Fluc into Balb/c mice
(Circ-Fluc is optimized with the best codon sequence and contains different IRES sequences)
