TRIzol Reagent is a ready-to-use total RNA extraction reagent that can rapidly extract total RNA from various tissues or cells of humans, animals, plants, yeast, and bacteria. It provides good separation results for small amounts of tissue (50–100 mg) and 5×10^6 cells, as well as for large amounts of tissue (≥1 g) and cells (>10^7).
While samples are thoroughly lysed inTRIzol Reagent, RNA integrity is maximally preserved. After centrifugation with added chloroform, the solution separates into three phases: an upper colorless aqueous phase, an interphase, and a lower red organic phase, with RNA localized in the aqueous phase. After collecting the aqueous phase, total RNA can be recovered by isopropanol precipitation.
Applications
The extracted totalRNA has good integrity, with no protein or DNA contamination, and can be used for RT-PCR, Real-time RT-PCR, Northern blot, Dot Blot, in vitro translation and other molecular biology experiments.
Product Features
·High quality: high purity of recovered RNA, good integrity, with no protein or other contaminant contamination;
·Wide applicability: broadly suitable for various animal, plant, and microbial samples, and easily handles all types of cultured cells;
·High efficiency: simple operation, high RNA recovery efficiency, extraction process takes only about 30 minutes;
·No contamination: the entire kit is treated RNase-Free;
·High cost-effectiveness: quality and yield comparable to overseas competitors, but at a more favorable price.
Shipping and Storage
Transport with ice packs.Store protected from light at 2~8°C, shelf life 12 months.
