Gisea BioTaqMan probe method for mycoplasma detection, based on TaqMan qPCR, targets the conserved region sequence of mycoplasma 16S rRNA, with specific primers and a fluorescent probe (FAM-labeled) designed for detection of mycoplasma DNA. This kit can directly use biological materials such as cell culture supernatant or serum as templates and can detect up to 16 common and uncommon mycoplasma types. The kit contains a dUTP/UNG enzyme system to prevent false positives caused by aerosol contamination of PCR products, greatly improving detection accuracy.
Detectable mycoplasma types
|
M. orale* |
M. mycoides |
M. capricolum |
M. arthritidis |
|
M. gallisepticum |
M. hominis* |
M. hyorhinis* |
M. penetrans |
|
M. pirum* |
M. salivarium* |
M. synoviae |
M. arginini* |
|
M. fermentans* |
M. pneumoniae |
M. genitalium |
A. laidlawii* |
*: The most common mycoplasma species contaminating cultured cells.
Product features
1. Simple operation: directly use 1 μL of cell culture supernatant or serum for detection, no DNA extraction required.
2. High detection sensitivity, high accuracy, tolerant to the effects of penicillin and streptomycin or other inhibitors.
3. High specificity: only amplifies Mycoplasma DNA and will not amplify bacterial, fungal, or eukaryotic cell DNA.
4. Rapid detection: results can be determined in as little as 1 hour.
5. Complete reaction system: includes negative control and positive control reaction systems.
6. Positive control sample is non-infectious.
Customer Article
1. Nikfarjam L, Farzaneh P. Prevention and detection of Mycoplasma contamination in cell culture. Cell J. 2012 Winter;13(4):203-12.
2. Molla Kazemiha V, Bonakdar S, Amanzadeh A, Azari S, Memarnejadian A, Shahbazi S, et al., Real-time PCR assay is superior to other methods for the detection of mycoplasma contamination in the cell lines of the National Cell Bank of Iran. Cytotechnology. 2016 Aug;68(4):1063-80.
Q & A
Precautions
1. Standardize testing operations; wear lab coat, gloves, and a mask,
2. Prepare reaction mixtures, process and load samples, and perform PCR amplification in separate areas to avoid cross-contamination.
